Journal: Nature Communications
Article Title: Strain dynamics of contaminating bacteria modulate the yield of ethanol biorefineries
doi: 10.1038/s41467-024-49683-2
Figure Lengend Snippet: A Ethanol yield from pairwise fermentations of industrial yeast strain PE-2 with 3 L. fermentum industrial isolates (blue) and the 5 most abundant bacteria in the bioethanol production microbiome, compared to standalone fermentation (white). Ethanol yields were enhanced by L. amylovorus , P. claussenii and L. buchneri , and reduced only by L. fermentum strain ( C ) (* p < 0.05). For all experiments, n = 3 biologically independent samples. Data are presented as mean values +/- SD. Final ethanol yields were compared by multiple t- test (statistical significance analysis with alpha value of 0.05). B Cladogram of the 3 L. fermentum isolates and other published L. fermentum genomes, based on the alignment of 40 bacterial marker genes. L. fermentum strains broadly cluster into 4 groups; strain ( C ) belongs to a different clade than strains ( A , B ). Internal nodes are labelled with bootstrap values from 500 resamplings. Image generated using MEGAX, with the triangular root replaced for visual clarity. C Metabolite profiles measured from the supernatant of the 3 L. fermentum industrial isolates. Strains A and B showed similar production of acetate, lactate and ethanol. Strain C (dark blue, right) produced no ethanol and almost twice as much lactate as strains A and B. Source data are provided as a Source Data file.
Article Snippet: Strains of Pediococcus claussenii , Lactobacillus helveticus, Lactobacillus buchneri and Zymomonas mobilis were purchased from ATCC (Manassas, VA, USA).
Techniques: Bacteria, Marker, Generated, Produced